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spaac reagent  (Eppendorf AG)


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    Structured Review

    Eppendorf AG spaac reagent
    Spaac Reagent, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 199205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spaac+reagent/Eppendorf/pmc11773117__ANIE___64___e202417620___s001-199-15-32
    Average 99 stars, based on 199205 article reviews
    spaac reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Conjugation Assay:

    Article Title: Site‐Specific Quadruple‐Functionalised Antibodies
    Article Snippet: S6.8 MTGase-mediated conjugation Antibody solution (10 μL, 17 mg/mL in PBS) was incubated with 11-Azido-3,6,9-trioxaundecan-1amine (termed AzidoLinker) (80 equiv., 0.8 μL of 0.1M stock solution, SigmaAldrich, 17758) and microbial transglutaminase (1 unit, SigmaAldrich, SAE0159) overnight at 25 °C in a 0.5 mL Eppendorf. .. S6.9 SPAAC conjugation Antibody solution (100 μL-2 mL, 1 mg/mL in PBS) was incubated with SPAAC reagent (5 equiv., 0.33- 6.66 μL of 10 mM DMSO stock) in a clean 0.5 mL Eppendorf at 25 °C for 1 h. S6.10 Maleimide conjugation Antibody solution (300-2000 μL, 1 mg/mL in PBS) was incubated with DL488-Mal (10 equiv., 3.3-22.2 μL of 5 mM DMSO stock) in a clean 0.5 mL Eppendorf at 25 °C for 1 h. S6.11 Decysteinylation and rebridging Antibody solution (750 μL, 4 mg/mL in PBS) was incubated with DTT (SigmaAldrich, 10197777001, 10 equiv., 20 μL of 10 mM stock solution) at 37 °C for 1 hour in a 1.5 mL Eppendorf. .. The reduced antibody solution was then mixed and incubated with dehydroascorbic acid (SigmaAldrich, 261556, 20 equiv., 40 μL of 10 mM DMSO stock solution) in a 1.5 mL Eppendorf at 25 °C for 45 minutes.

    Incubation:

    Article Title: Site‐Specific Quadruple‐Functionalised Antibodies
    Article Snippet: S6.8 MTGase-mediated conjugation Antibody solution (10 μL, 17 mg/mL in PBS) was incubated with 11-Azido-3,6,9-trioxaundecan-1amine (termed AzidoLinker) (80 equiv., 0.8 μL of 0.1M stock solution, SigmaAldrich, 17758) and microbial transglutaminase (1 unit, SigmaAldrich, SAE0159) overnight at 25 °C in a 0.5 mL Eppendorf. .. S6.9 SPAAC conjugation Antibody solution (100 μL-2 mL, 1 mg/mL in PBS) was incubated with SPAAC reagent (5 equiv., 0.33- 6.66 μL of 10 mM DMSO stock) in a clean 0.5 mL Eppendorf at 25 °C for 1 h. S6.10 Maleimide conjugation Antibody solution (300-2000 μL, 1 mg/mL in PBS) was incubated with DL488-Mal (10 equiv., 3.3-22.2 μL of 5 mM DMSO stock) in a clean 0.5 mL Eppendorf at 25 °C for 1 h. S6.11 Decysteinylation and rebridging Antibody solution (750 μL, 4 mg/mL in PBS) was incubated with DTT (SigmaAldrich, 10197777001, 10 equiv., 20 μL of 10 mM stock solution) at 37 °C for 1 hour in a 1.5 mL Eppendorf. .. The reduced antibody solution was then mixed and incubated with dehydroascorbic acid (SigmaAldrich, 261556, 20 equiv., 40 μL of 10 mM DMSO stock solution) in a 1.5 mL Eppendorf at 25 °C for 45 minutes.



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    spaac reagent  (Eppendorf AG)
    99
    Eppendorf AG spaac reagent
    Spaac Reagent, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spaac+reagent/Eppendorf/pmc11773117__ANIE___64___e202417620___s001-199-15-32
    Average 99 stars, based on 1 article reviews
    spaac reagent - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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